Rainbow trout were exposed to low concentrations of TCDD (0.05 - 0.8 ng/L tetrachlorodibenzo-p-dioxin) and TCDF (1-16 ng/L tetrachlorodibenzofuran) for 28 or 60 days in a flow-through diluter system with 10L exposure tanks. The effects of chronic exposure to these compounds on the survival, growth, and behavior of the trout were evaluated. Surviving fish were then held in clean flowing water for up to 14 or 30 additional days to measure bioaccumulation and depuration of the chemicals. A radio-labeled TCDD technique was employed to detect and verify the low water concentrations of this compound.
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